New heat-stable acid-phosphatase test for differentiation of mycobacteria.

The heat stable acid phosphatase activity of Mycobacteria.

Fluorimetric assay of mycobacterial group-specific hydrolase enzymes.

Fluorigenic substrates prepared from 4-methylumbelliferone provide a simple, convenient method for detecting and assaying group-specific hydrolase activity in small quantities of whole mycobacteria. Physico-chemical properties of the enzymes such as pH dependence and heat stability may also be studied. A technique is described for studying glycosidase, hexosaminidase, acid phosphatase, and acyl...

Comparison of Proliferation and Osteoblast Differentiation of Marrow-Derived Mesenchymal Stem Cells on Nano- and Micro-Hydroxyapatite Contained Composite Scaffolds

Bones constructed by tissue engineering are being considered as valuable materials to be used for regeneration of large defects in natural bone. In an attempt to prepare a new bone construct, in this study, proliferation and bone differentiation of marrow-derived mesenchymal stem cells (MSCs) on our recently developed composite scaffolds of nano-, micro-hydroxyapatite/ poly(l-lactic acid) were ...

Effect of heat treatment on H2O2/HCl etched pure titanium dental implant: An in vitro study

BACKGROUND Surface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H2O2/HCl-treated Ti implants in cell attachment, proliferation and osteoblastic differentiation. MATERIAL/METHODS Sandblasted, dual acid-etch...

Evaluation of the Effect of Platelet-Rich Plasma on Proliferation and Differentiation of Human Dental Pulp Stem Cells with or without Ga-Al-As Laser

Background Recently, the clinical use of low power lasers has increased, and it is said that wound healing is accelerated by their irradiation. The aim of this study was evaluation of the effect of platelet-rich plasma on proliferation and differentiation of human dental pulp stem cells with or without Ga-Al-As laser. Methods: In this experimental study, human lower third molar dental pulp c...